Journal article
2D-DIGE analysis of sera from transgenic mouse models reveals novel candidate protein biomarkers for human gastric cancer
MAS Penno, M Klingler-Hoffmann, JA Brazzatti, A Boussioutas, T Putoczki, M Ernst, P Hoffmann
Journal of Proteomics | ELSEVIER SCIENCE BV | Published : 2012
Abstract
The gp130F/F genetically engineered mouse (GEM) model reproducibly and predictably develops a gastric adenoma phenotype resembling the primary lesions of human intestinal-type gastric cancer (GC). Accordingly, changes to the serum proteome of gp130F/F mice may uncover early-stage GC biomarkers. Here, we have employed several double and compound mutant GEM strains that display distinct phenotypes with respect to gastric tumour load and inflammatory response, thereby mimicking different states of inflammation-associated early-stage GC in humans. This allowed us to distinguish between proteomic changes associated with tumourigenesis rather than confounding systemic inflammation. The comparative..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors wish to thank Melanie Asquith for technical assistance with serum collection and the staff of the animal facility at the Ludwig Institute for Cancer Research for husbandry of mice, A/Prof Martin K. Oehler (University of Adelaide/Royal Adelaide Hospital) for collection of the control serum, and Dr. Wade Hines (Australian Wine Research Institute) for critical revision of the manuscript. This work was supported by the Australian National Health and Medical Research Council (NHMRC) grants 433617, 487922 and APP1010728, by a NHMRC Senior Research Fellowship to M.E, and by funds from the Operational Infrastructure Support Program provided by the Victorian Government.